Yulia Carvalho

• Owned laboratory quality assurance - improving workflow structure, data traceability, and documentation standards through SOP creation and process guidance.
• Participated in the implementation and configuration of a LIMS tailored to laboratory needs - defining required sections, records, and operational data structures.
• Migrated legacy laboratory records into the LIMS, including Excel trackers, equipment maintenance logs, and related operational data.
• Authored the SOP for LIMS use to standardize system adoption, data handling, and operational consistency across the lab.
• Applied data-driven resource tracking to monitor consumable usage, forecast needs, optimize inventory, and reduce waste.
• Managed core lab operations - equipment maintenance, supplier coordination, and safety compliance - supported by structured logs and monitoring.
• Coordinated biological sample inflow, processing, and logistics, ensuring accurate registration, tracking, and reporting.

• Managed the planning, coordination, and execution of 200,000+ COVID-19 diagnostic tests under enhanced BSL-II conditions, ensuring accurate tracking, prioritization, and timely reporting.
• Performed qPCR result analysis and prepared structured reports for patients and external entities with strong focus on data accuracy, completeness, and traceability.
• Contributed to the design and optimization of laboratory protocols by calculating minimum tolerances, analyzing consumable usage patterns, and streamlining workflows - reducing cost without compromising diagnostic quality.
• Applied domain knowledge, basic statistics, and digital tools to validate and maintain SINAVE data, ensuring data integrity, consistency, and up-to-date records for surveillance and reporting.

• Oversaw lab management and maintenance while supporting molecular cloning, virus production, and mouse colony genotyping - ensuring documentation, traceability, and protocol compliance.
• Collected, organized, and analyzed experimental data from confocal microscopy and molecular assays, using spreadsheets and image-analysis tools to quantify results, assess variability, and support statistically sound conclusions.
• Contributed to individual scientific projects and collaborated with PhD students and postdocs - helping design experiments, interpret data, and troubleshoot protocols based on quantitative readouts and controls.
• Helped prepare figures, datasets, and method descriptions for internal reports, presentations, and publications, reinforcing a rigorous, reproducible, and data-driven approach.

Project I: “Epigenetics of stem cells: uncovering the role of noncoding RNAs” - identification and characterization of histone-H3-bound long noncoding RNAs in mouse stem cells.
Project II: “Identification and characterization of long noncoding RNAs in murine heart” - screening for positionally-conserved heart-expressed, -enriched or -specific lncRNAs and their molecular and functional characterization.

• Handled large, complex sequencing and microarray datasets, integrating multiple experimental readouts.
• Applied statistical methods to assess significance, validate findings, and interpret high-dimensional data in a robust, reproducible way.
• Prepared and delivered presentations at scientific conferences and internal meetings, clearly communicating complex results, methods, and limitations to diverse audiences.
• Contributed to peer-reviewed publications by designing and performing experiments, analyzing data, and documenting results - reinforcing a rigorous, evidence-based, data-driven research approach.

• Researched lncRNAs in embryonic stem cells; performed global transcriptome analysis during cardiac differentiation.
• Co-authored a paper in Nucleic Acids Research (2012) on “Noncoder,” a web interface for exon-array detection of lncRNAs.

• Recombinant viral hepatitis vaccine development.